anti-rap 1a Search Results


anti rap1a antibody  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti rap1a antibody
Figure 3 FTI-277 enhances the ability of TGFb1 to induce transcription. (A) Panc-1 cells were cotransfected with 2 mg of p3TP-Lux reporter construct and 0.5 mg of pCMV-bgal. Fifteen hours post-transfection, cells were incubated with vehicle (DMSO), FTI-277 (15 mM), and/or TGFb1 (200 pM) for 24 h. The fold induction was calculated by dividing the luciferase activity values of FTI-277- or TGFb1-treated samples by that of vehicle-treated control. Data are means+s.e. for six experiments. (B) FTI-277 inhibits Lamin B farnesylation but not <t>Rap1A</t> geranylgeranylation. Cell lysates from experiment (A) above were analysed by SDS ± PAGE followed by immunoblotting with an anti-Lamin B or anti-Rap1A antibody. Lamin B is a substrate for farnesyltransferase and thus, its farnesylation was inhibited by FTI-277 (lanes 2 and 4). However, FTI-277 had no eect on Rap1A (lanes 2 and 4), which is a substrate for geranylgeranyl- transferase I. U for unprenylated and P for prenylated form. (C) Nuclear extracts (NE) from FTI-277- and TGFb1-treated samples were used in EMSA to determine the status of nuclear complexes that bound TRE sequence, similar to that present in p3TP-Lux reporter. The binding reactions contained NE from vehicle- treated samples and either 1006 of wild type competitor, TRE (lane 1) or 1006 of an unrelated oligonucleotide (lane 2). In addition to NE from speci®c samples, as indicated on top of the ®gure, the binding reactions contained either a normal rabbit antibody (lanes 3 ± 6) or an anti-AP1 antibody (lanes 7 ± 10). Note, cotreatment of cells with both FTI-277 and TGFb led to a higher level of TRE-bound complexes (lane 6) that were supershifted with an anti-AP1 antibody (lane 10). Data is representative of four independent experiments
Anti Rap1a Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-rap+1a/pm11114730-157-20-25?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
anti rap1a antibody - by Bioz Stars, 2026-07
93/100 stars
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antirap 1a/b rabbit polyclonal antibodies  (GenScript corporation)
90
GenScript corporation antirap 1a/b rabbit polyclonal antibodies
Figure 3 FTI-277 enhances the ability of TGFb1 to induce transcription. (A) Panc-1 cells were cotransfected with 2 mg of p3TP-Lux reporter construct and 0.5 mg of pCMV-bgal. Fifteen hours post-transfection, cells were incubated with vehicle (DMSO), FTI-277 (15 mM), and/or TGFb1 (200 pM) for 24 h. The fold induction was calculated by dividing the luciferase activity values of FTI-277- or TGFb1-treated samples by that of vehicle-treated control. Data are means+s.e. for six experiments. (B) FTI-277 inhibits Lamin B farnesylation but not <t>Rap1A</t> geranylgeranylation. Cell lysates from experiment (A) above were analysed by SDS ± PAGE followed by immunoblotting with an anti-Lamin B or anti-Rap1A antibody. Lamin B is a substrate for farnesyltransferase and thus, its farnesylation was inhibited by FTI-277 (lanes 2 and 4). However, FTI-277 had no eect on Rap1A (lanes 2 and 4), which is a substrate for geranylgeranyl- transferase I. U for unprenylated and P for prenylated form. (C) Nuclear extracts (NE) from FTI-277- and TGFb1-treated samples were used in EMSA to determine the status of nuclear complexes that bound TRE sequence, similar to that present in p3TP-Lux reporter. The binding reactions contained NE from vehicle- treated samples and either 1006 of wild type competitor, TRE (lane 1) or 1006 of an unrelated oligonucleotide (lane 2). In addition to NE from speci®c samples, as indicated on top of the ®gure, the binding reactions contained either a normal rabbit antibody (lanes 3 ± 6) or an anti-AP1 antibody (lanes 7 ± 10). Note, cotreatment of cells with both FTI-277 and TGFb led to a higher level of TRE-bound complexes (lane 6) that were supershifted with an anti-AP1 antibody (lane 10). Data is representative of four independent experiments
Antirap 1a/B Rabbit Polyclonal Antibodies, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-rap+1a/10__1074_slash_jbc__m109__015362-71-0-16?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
antirap 1a/b rabbit polyclonal antibodies - by Bioz Stars, 2026-07
90/100 stars
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mouse anti-ras-related protein rap 1a  (Becton Dickinson)
90
Becton Dickinson mouse anti-ras-related protein rap 1a
Figure 3 FTI-277 enhances the ability of TGFb1 to induce transcription. (A) Panc-1 cells were cotransfected with 2 mg of p3TP-Lux reporter construct and 0.5 mg of pCMV-bgal. Fifteen hours post-transfection, cells were incubated with vehicle (DMSO), FTI-277 (15 mM), and/or TGFb1 (200 pM) for 24 h. The fold induction was calculated by dividing the luciferase activity values of FTI-277- or TGFb1-treated samples by that of vehicle-treated control. Data are means+s.e. for six experiments. (B) FTI-277 inhibits Lamin B farnesylation but not <t>Rap1A</t> geranylgeranylation. Cell lysates from experiment (A) above were analysed by SDS ± PAGE followed by immunoblotting with an anti-Lamin B or anti-Rap1A antibody. Lamin B is a substrate for farnesyltransferase and thus, its farnesylation was inhibited by FTI-277 (lanes 2 and 4). However, FTI-277 had no eect on Rap1A (lanes 2 and 4), which is a substrate for geranylgeranyl- transferase I. U for unprenylated and P for prenylated form. (C) Nuclear extracts (NE) from FTI-277- and TGFb1-treated samples were used in EMSA to determine the status of nuclear complexes that bound TRE sequence, similar to that present in p3TP-Lux reporter. The binding reactions contained NE from vehicle- treated samples and either 1006 of wild type competitor, TRE (lane 1) or 1006 of an unrelated oligonucleotide (lane 2). In addition to NE from speci®c samples, as indicated on top of the ®gure, the binding reactions contained either a normal rabbit antibody (lanes 3 ± 6) or an anti-AP1 antibody (lanes 7 ± 10). Note, cotreatment of cells with both FTI-277 and TGFb led to a higher level of TRE-bound complexes (lane 6) that were supershifted with an anti-AP1 antibody (lane 10). Data is representative of four independent experiments
Mouse Anti Ras Related Protein Rap 1a, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-rap+1a/pmc02947716-279-65-70?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
mouse anti-ras-related protein rap 1a - by Bioz Stars, 2026-07
90/100 stars
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anti-rap 1a/b rabbit polyclonal antibodies  (GenScript corporation)
90
GenScript corporation anti-rap 1a/b rabbit polyclonal antibodies
Figure 3 FTI-277 enhances the ability of TGFb1 to induce transcription. (A) Panc-1 cells were cotransfected with 2 mg of p3TP-Lux reporter construct and 0.5 mg of pCMV-bgal. Fifteen hours post-transfection, cells were incubated with vehicle (DMSO), FTI-277 (15 mM), and/or TGFb1 (200 pM) for 24 h. The fold induction was calculated by dividing the luciferase activity values of FTI-277- or TGFb1-treated samples by that of vehicle-treated control. Data are means+s.e. for six experiments. (B) FTI-277 inhibits Lamin B farnesylation but not <t>Rap1A</t> geranylgeranylation. Cell lysates from experiment (A) above were analysed by SDS ± PAGE followed by immunoblotting with an anti-Lamin B or anti-Rap1A antibody. Lamin B is a substrate for farnesyltransferase and thus, its farnesylation was inhibited by FTI-277 (lanes 2 and 4). However, FTI-277 had no eect on Rap1A (lanes 2 and 4), which is a substrate for geranylgeranyl- transferase I. U for unprenylated and P for prenylated form. (C) Nuclear extracts (NE) from FTI-277- and TGFb1-treated samples were used in EMSA to determine the status of nuclear complexes that bound TRE sequence, similar to that present in p3TP-Lux reporter. The binding reactions contained NE from vehicle- treated samples and either 1006 of wild type competitor, TRE (lane 1) or 1006 of an unrelated oligonucleotide (lane 2). In addition to NE from speci®c samples, as indicated on top of the ®gure, the binding reactions contained either a normal rabbit antibody (lanes 3 ± 6) or an anti-AP1 antibody (lanes 7 ± 10). Note, cotreatment of cells with both FTI-277 and TGFb led to a higher level of TRE-bound complexes (lane 6) that were supershifted with an anti-AP1 antibody (lane 10). Data is representative of four independent experiments
Anti Rap 1a/B Rabbit Polyclonal Antibodies, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-rap+1a/pmc02757186-70-0-16?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
anti-rap 1a/b rabbit polyclonal antibodies - by Bioz Stars, 2026-07
90/100 stars
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anti rap 1a sc 1482 antiserum  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti rap 1a sc 1482 antiserum
Figure 3 FTI-277 enhances the ability of TGFb1 to induce transcription. (A) Panc-1 cells were cotransfected with 2 mg of p3TP-Lux reporter construct and 0.5 mg of pCMV-bgal. Fifteen hours post-transfection, cells were incubated with vehicle (DMSO), FTI-277 (15 mM), and/or TGFb1 (200 pM) for 24 h. The fold induction was calculated by dividing the luciferase activity values of FTI-277- or TGFb1-treated samples by that of vehicle-treated control. Data are means+s.e. for six experiments. (B) FTI-277 inhibits Lamin B farnesylation but not <t>Rap1A</t> geranylgeranylation. Cell lysates from experiment (A) above were analysed by SDS ± PAGE followed by immunoblotting with an anti-Lamin B or anti-Rap1A antibody. Lamin B is a substrate for farnesyltransferase and thus, its farnesylation was inhibited by FTI-277 (lanes 2 and 4). However, FTI-277 had no eect on Rap1A (lanes 2 and 4), which is a substrate for geranylgeranyl- transferase I. U for unprenylated and P for prenylated form. (C) Nuclear extracts (NE) from FTI-277- and TGFb1-treated samples were used in EMSA to determine the status of nuclear complexes that bound TRE sequence, similar to that present in p3TP-Lux reporter. The binding reactions contained NE from vehicle- treated samples and either 1006 of wild type competitor, TRE (lane 1) or 1006 of an unrelated oligonucleotide (lane 2). In addition to NE from speci®c samples, as indicated on top of the ®gure, the binding reactions contained either a normal rabbit antibody (lanes 3 ± 6) or an anti-AP1 antibody (lanes 7 ± 10). Note, cotreatment of cells with both FTI-277 and TGFb led to a higher level of TRE-bound complexes (lane 6) that were supershifted with an anti-AP1 antibody (lane 10). Data is representative of four independent experiments
Anti Rap 1a Sc 1482 Antiserum, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-rap+1a/pmc05017209-107-5-10?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
anti rap 1a sc 1482 antiserum - by Bioz Stars, 2026-07
96/100 stars
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rap1a polyclonal antibody, alexa fluor 488 conjugated  (Bioss)
N/A
Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together
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rap1a polyclonal antibody, hrp conjugated  (Bioss)
N/A
Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together
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rap1a polyclonal antibody, alexa fluor 680 conjugated  (Bioss)
N/A
Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together
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rap1a antibody 5f8g2 biotin  (Novus Biologicals)
N/A
The RAP1A Antibody 5F8G2 Biotin from Novus Biologicals is a mouse monoclonal antibody to RAP1A This antibody reacts with human The RAP1A Antibody 5F8G2 Biotin has been validated for the following applications Western Blot ELISA
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rap1a antibody rap1 4c8 1 alexa fluor« 700  (Novus Biologicals)
N/A
The RAP1A Antibody RAP1 4C8 1 Alexa Fluor« 700 from Novus Biologicals is a mouse monoclonal antibody to RAP1A This antibody reacts with human mouse The RAP1A Antibody RAP1 4C8 1 Alexa Fluor« 700 has
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rap1a antibody c terminal region  (Abcepta)
N/A
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goat anti-fem1a / aprap antibody (oaeb01989)  (Aviva Systems)
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Image Search Results


Figure 3 FTI-277 enhances the ability of TGFb1 to induce transcription. (A) Panc-1 cells were cotransfected with 2 mg of p3TP-Lux reporter construct and 0.5 mg of pCMV-bgal. Fifteen hours post-transfection, cells were incubated with vehicle (DMSO), FTI-277 (15 mM), and/or TGFb1 (200 pM) for 24 h. The fold induction was calculated by dividing the luciferase activity values of FTI-277- or TGFb1-treated samples by that of vehicle-treated control. Data are means+s.e. for six experiments. (B) FTI-277 inhibits Lamin B farnesylation but not Rap1A geranylgeranylation. Cell lysates from experiment (A) above were analysed by SDS ± PAGE followed by immunoblotting with an anti-Lamin B or anti-Rap1A antibody. Lamin B is a substrate for farnesyltransferase and thus, its farnesylation was inhibited by FTI-277 (lanes 2 and 4). However, FTI-277 had no eect on Rap1A (lanes 2 and 4), which is a substrate for geranylgeranyl- transferase I. U for unprenylated and P for prenylated form. (C) Nuclear extracts (NE) from FTI-277- and TGFb1-treated samples were used in EMSA to determine the status of nuclear complexes that bound TRE sequence, similar to that present in p3TP-Lux reporter. The binding reactions contained NE from vehicle- treated samples and either 1006 of wild type competitor, TRE (lane 1) or 1006 of an unrelated oligonucleotide (lane 2). In addition to NE from speci®c samples, as indicated on top of the ®gure, the binding reactions contained either a normal rabbit antibody (lanes 3 ± 6) or an anti-AP1 antibody (lanes 7 ± 10). Note, cotreatment of cells with both FTI-277 and TGFb led to a higher level of TRE-bound complexes (lane 6) that were supershifted with an anti-AP1 antibody (lane 10). Data is representative of four independent experiments

Journal: Oncogene

Article Title: Inhibition of farnesyltransferase increases TGFbeta type II receptor expression and enhances the responsiveness of human cancer cells to TGFbeta.

doi: 10.1038/sj.onc.1203920

Figure Lengend Snippet: Figure 3 FTI-277 enhances the ability of TGFb1 to induce transcription. (A) Panc-1 cells were cotransfected with 2 mg of p3TP-Lux reporter construct and 0.5 mg of pCMV-bgal. Fifteen hours post-transfection, cells were incubated with vehicle (DMSO), FTI-277 (15 mM), and/or TGFb1 (200 pM) for 24 h. The fold induction was calculated by dividing the luciferase activity values of FTI-277- or TGFb1-treated samples by that of vehicle-treated control. Data are means+s.e. for six experiments. (B) FTI-277 inhibits Lamin B farnesylation but not Rap1A geranylgeranylation. Cell lysates from experiment (A) above were analysed by SDS ± PAGE followed by immunoblotting with an anti-Lamin B or anti-Rap1A antibody. Lamin B is a substrate for farnesyltransferase and thus, its farnesylation was inhibited by FTI-277 (lanes 2 and 4). However, FTI-277 had no eect on Rap1A (lanes 2 and 4), which is a substrate for geranylgeranyl- transferase I. U for unprenylated and P for prenylated form. (C) Nuclear extracts (NE) from FTI-277- and TGFb1-treated samples were used in EMSA to determine the status of nuclear complexes that bound TRE sequence, similar to that present in p3TP-Lux reporter. The binding reactions contained NE from vehicle- treated samples and either 1006 of wild type competitor, TRE (lane 1) or 1006 of an unrelated oligonucleotide (lane 2). In addition to NE from speci®c samples, as indicated on top of the ®gure, the binding reactions contained either a normal rabbit antibody (lanes 3 ± 6) or an anti-AP1 antibody (lanes 7 ± 10). Note, cotreatment of cells with both FTI-277 and TGFb led to a higher level of TRE-bound complexes (lane 6) that were supershifted with an anti-AP1 antibody (lane 10). Data is representative of four independent experiments

Article Snippet: Brie ̄y, aliquots of cell lysate were analysed by SDS±PAGE followed by immunoblotting with anti-Lamin B antibody or, as control, anti-Rap1A antibody (sc-6216, sc-1482, respectively, SantaCruz Biotechnology).

Techniques: Construct, Transfection, Incubation, Luciferase, Activity Assay, Control, SDS Page, Western Blot, Sequencing, Binding Assay